An experiment to look at the effect of 1 factor on an enzyme

Aim To design and carry out an audition to side at one factor which affects the enzyme catalase. Catalase is involved in the breakdown of total heat Peroxide into water and atomic name 8. 2H202 _ 2H20 + 02 It can be found in most cells, barely it is mostly concentrated in the liver, kidney and genus Tuber cells of plants (e.g. spud). Hypothesis I predict that as the temperature increases the atomic number 8 produced freeze increase until a point (optimum temperature), then pull up stakes devolve until no oxygen is produced ( due to denaturation ) Justification All enzymes set out optimum conditions, at which they work best. therefrom in my experiment there lead be an optimum temperature i.e. the temperature where oxygen is abandoned off most rapidly. I stated that at a temperature no oxygen would be produced. This is because I cogitate the enzyme provide denature due to the high temperature ( ie the tertiary social system go out change ). Du e to the denaturation of the enzyme, no oxygen leave alone be produced. I also feel it is important to stick note that the H2O2 is an unstable compound and at high temperatures (100aC) it will disintegrate by itself.

Apparatus For my experiment I will need the chase frame-up: h Conical flaskful with side on delivery outlet h ladened fitting bung for the conic flask h preservation tube h Gas-syringe h Water bathroom h Retort stand h Boss and clamp I will set the apparatus up in the hobby way ¡V Control of variables FactorControl TemperatureI have elect to substitute this factor using an electronic wat er bath in my experiment. ConcentrationI wil! l use the substrate from the same bottleful without dilution. I will take my cuttings of potato from the same region. Surface AreaI will use the same cork borer and cut every(prenominal) of the potato to the... If you want to get a full essay, ordination it on our website: BestEssayCheap.com

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